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KMID : 0644120090220010015
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Journal of Oriental Gynecology
2009 Volume.22 No. 1 p.15 ~ p.30
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Neuroprotective Effect of Yukul-tang against the Oxidative Stress
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Jung Sun-Hyung
Lee Jin-Moo
Lee Chang-Hoon
Cho Jung-Hoon
Jang Jun-Bock
Lee Kyung-Sub
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Abstract
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Purpose: In this rapidly aging society, the research and development of traditional oriental medicine treatment is one of the critical factors to protect the increasing neuro-degenerative disorders. In this study, we wanted to verify the effect of Yukul-tang (YUT) on neuro-degenerative disease model by assessing the antioxidant and anti-inflammation effects.
Methods: To assess the antioxidant effects of YUT, we carried out DPPH radical and ABTS radical scavenging assays and determined the total polyphenolic contents in YUT. To evaluate the neuro-protective effects of YUT, we performed the MTT and ROS assays and TH immunohistochemistry, NO and TNF- assays in SH-SY5Y or mesencephalic dopaminergic neurons damaged by 6-OHDA.
Results: The treatment of YUT showed eliminating effects on DPPH radical and ABTS radical. it showed deterring effects on ROS, NO and TNF- and protecting effects on TH-positive cell in SH-SY5Y cells or mesencephalic dopaminergic neurons. Especially in the case of the treatment of YUT with 0.2ug/mL + 6-OHDA 10uM, the protective effect on dopaminergic neurons was most outstanding.
Conclusion: In this study, we have demonstrated that YUT has an antioxidant effect and a neuro-protective effect on neuro-degenerative disease model caused by neurotoxin such as 6-OHDA. The results of our present study suggest that YUT can be useful agent to prevent and to treat neuro-degenerative diseases.
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KEYWORD
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Yukul-tang (YUT), 6-OHDA, Antioxidant effect, Protection of Dopaminergic neurons
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